A-10
ACC 132
Cell line:
A-10
DSMZ no.:
ACC 132
Species:
rat (Rattus norvegicus)
Cell type:
smooth muscle
Origin:
established from the thoracic aorta of a DB1X embryonic rat in 1976; cells were described in the literature to display properties characteristic of smooth muscle cells
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
80% DMEM + 20% h.i. FBS
Subculture:
seed out at 1 x 106 cells/25 cm2; split confluent culture 1:2 to 1:3 once or twice a week using trypsin/EDTA; after thawing, cells may contain a significant amount of granulae; confluent cultures look more transparent
Incubation:
at 37 °C with 10% CO2
Doubling time:
ca. 70-100 hours
Harvest:
about 1-2 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Rattus norvegicus species.
Cytogenetics:
rat karyotype
Viruses:
ELISA: reverse transcriptase positive; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list