EM-3
ACC 134
Cell line:
EM-3
DSMZ no.:
ACC 134
Species:
human (Homo sapiens)
Cell type:
chronic myeloid leukemia in blast crisis
Origin:
established from the bone marrow of a 5-year-old girl with Philadelphia chromosome-positive CML (chronic myeloid leukemia) in second relapse in 1980 (after bone marrow transplantation day +47); sister cell line of EM-2 (DSM ACC 135)
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
maintain at 0.5-1.5 x 106 cells/ml; subculture at a 1:2 to 1:3 dilution every 48 hours; seed out at ca. 0.5 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 40 hours
Harvest:
grows up to about 2 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
CD3 -, CD4 -, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, HLA-DR -
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypertriploid karyotype - 73(69-77)<3n>X, -X, -X, t(9;22)(q34;q11), i(17q)
Molec. Genetics:
expression of fusion gene BCR-ABL1 e14-a2 (b3-a2) confirmed by RT-PCR
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list