BPH-1
ACC 143
Cell line:
BPH-1
DSMZ no.:
ACC 143
Species:
human (Homo sapiens)
Cell type:
benign prostate hyperplasia
Origin:
prostate epithelial cells from a 68-year-old man with benign prostate hyperplasia; cells were immortalized with SV-40 large T-antigen; cells were described in the literature to express cytokeratins 8, 18, and 19 (but not 14) and to metabolize prostatic androgens
Reference(s):
Biosafety level:
1, GMO-S1
Risk assessment:
genetically modified organism, according to German and European legislation assigned to safety group S1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
80% RPMI 1640 + 20% h.i. FBS + 20 ng/ml testosterone + 5 µg/ml transferrin + 5 ng/ml sodium selenite + 5 µg/ml insulin
Subculture:
seed out at 0.5-1 x 106 cells in a 80cm2 flask; split confluent culture 1:3 to 1:8 every 3-7 days using trypsin/EDTA (cells should be incubated for more than 10 min with trypsin/EDTA at 37 °C)
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50 hours
Harvest:
cell harvest of ca. 3 x 106 cells/80cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypertriploid karyotype with 2.5% polyploidy - 75(69-79)<3n>XXY, -4, +5, +6, +7, -10, +11, -12, +15, +21, +3mar - +dmin - i(3q), add(7)(p11), i(8q), add(10)(p13) - sideline with add(13)(p12) and smaller 10p+ marker - matches published karyotype - carries large submetacentric 10p+ marker
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list