P3/NSI/1-AG4-1
ACC 145
Cell line:
P3/NSI/1-AG4-1
DSMZ no.:
ACC 145
Species:
murine (mouse) (Mus musculus)
Cell type:
myeloma
Origin:
P3/NSI/1-AG4-1 is a non-secreting clone of P3X63AG8 which is derived from P3K, the induced plasmacytoma MOPC-21; cells do not grow in HAT medium, extensively used in cell fusion studies; cells were described in the literature to synthesize kappa chain, but do not secrete free light chain
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
92.5-95% Iscove's MDM + 5-7.5% h.i. FBS + 2 mM L-glutamine
Subculture:
maintain at 0.1-0.8 x 106 cells/ml; split 1:3 to 1:10 every 1-3 days; initially after thawing, the viability may be reduced down to ca. 65%, but cells recover after about 1-2 days
Incubation:
at 37 °C with 7.5% CO2
Doubling time:
24 hours
Harvest:
maximal density at 1.2-1.4 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Cytogenetics:
hypertriploid murine karyotype with 2% polyploidy - 66(62-72)<3n> - 3-4 centric fusion markers present
Viruses:
ELISA: reverse transcriptase positive; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list