FLK-BLV-044
ACC 153
Cell line:
FLK-BLV-044
DSMZ no.:
ACC 153
Species:
ovine (sheep) (Ovis aries)
Cell type:
embryonal kidney cells
Origin:
established from the embryonal kidney cells of an ovine in 1976; cells are susceptible to infection with BLV (bovine leukemia virus) leading to the formation of syncythia
Reference(s):
Biosafety level:
1
Risk assessment:
The cell line is infected with bovine leukemia virus (BLV) and produces active viruses. The viruses are not pathogenic to humans, but may be pathogenic to animals.
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
90% MEM (with Earle's salts) + 10% h.i. FBS optional: + 850 mg/L NaHCO3 + 120 mg/L sodium pyruvate + 1x non-essential amino acids
Subculture:
seed out about 1-2 x 106 cells/80 cm2;
split confluent culture 1:2 to 1:8 every 3-5 days using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50 hours
Harvest:
cell harvest of about 4 x 106/80 cm2
Storage:
frozen with 82.5% medium, 10% FBS, 7.5% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Ovis aries species.
Viruses:
ELISA: reverse transcriptase positive; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list