PAB-122
ACC 166
Cell line:
PAB-122
DSMZ no.:
ACC 166
Species:
murine (mouse) (Mus musculus)
Cell type:
hybridoma (anti-p53)
Origin:
established by fusion of NS-1 myeloma cells and spleen cells from a male BALB/c mouse immunized with SV40-transformed, 3T3 clone 4 mouse cells; cells were reported to secrete an IgG2b antibody recognizing p53 protein present in nuclei of SV40-transformed cells
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
80% DMEM + 20% h.i. FBS
Subculture:
maintain at 0.5-5 x 105 cells/ml; split 1:5 every 2-3 days
Incubation:
at 37 °C with 5% CO2
Harvest:
maximal density at 1-2 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Viruses:
ELISA: reverse transcriptase negative
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list