BC3H1
ACC 171
Cell line:
BC3H1
DSMZ no.:
ACC 171
Species:
murine (mouse) (Mus musculus)
Cell type:
muscle cells from intracranial tumor
Origin:
established from the offspring of a C3H-mouse injected with nitrosoethylurea during pregnancy; cells show properties characteristic of muscle cells and were described in the literature to synthesize adenylate and creatine phosphokinases, tropomyosin and myosin
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
80% DMEM + 20 % h.i. FBS
Subculture:
split confluent culture 1:2 to 1:3 every week (cells grow very slowly) using trypsin/EDTA; cell numbers may be difficult to ascertain due to formation of clumps after detachment
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 70 hours
Harvest:
about 1-2 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Cytogenetics:
murine near-tetraploid karyotype - 74-82<4n> - 1-3 centric fusion markers and 1-2 dm present in most cells - does not correspond with published karyotype of 56 chromosomes
Viruses:
ELISA: reverse transcriptase positive
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list