Leibniz Institute DSMZ: Details

Cell line:

BTI-EAA

DSMZ no.:

ACC 176

Species:

insect - Estigmene acrea (Estigmene acrea)

Cell type:

Origin:

established from the larvae of the Acrea Moth (Estigmene acrea; family Arctiidae, order Lepidoptera) (Baerenspinner)

Reference(s):

not published

Biosafety level:

1

Permissions and restrictions:

A

DSMZ Cell Culture Data:

Morphology:

very large cells growing in suspension; most cells are round to oval, 10% have long processes and are spindle shaped; image; image; image; image; image; image

Medium:

80% TC 100 medium + 20% h.i. FBS

Subculture:

cells are difficult to culture and grow slowly! split saturated culture 1:4 to 1:5 every 4-5 days

Incubation:

no CO₂ at room temperature (20-25 °C)

Harvest:

cell harvest of about 0.5 x 10⁶ cells/ml

Storage:

frozen with 70% medium, 20% FBS, 10% DMSO

DSMZ Scientific Data:

Mycoplasma:

negative in DAPI, microbiological culture, RNA hybridization assays

Species validation:

Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Estigmene acrea species.

Supplied as:

Delivery form		Prices

Frozen culture		450,- €
Growing culture (please inquire for exact delivery time)		900,- €
DNA isolated from cell line (25 µg)		560,- €
DNA isolated from cell line (5 µg)		140,- €

see price list

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