TN-368
ACC 177
Cell line:
TN-368
DSMZ no.:
ACC 177
Species:
insect - Tiger moth (Trichoplusia ni)
Cell type:
larval cells
Origin:
established from the larvae of a tiger moth (species Trichoplusia ni = Autographa brassicae = Plusia ni, family Noctuidae, subfamily Plusiinae, order Lepidoptera)
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
80% TC 100 medium + 20% h.i. FBS
Subculture:
split saturated culture 1:4 to 1:10 every 2-3; maintain at 0.4-0.5 x 106 cells/ml; after thawing, cells do not grow during the first 2-4 days and viability may drop to about 10%; seed out at ca. 0.1 x 106 cells/ml
Incubation:
without CO2 at room temperature (20-25 °C)
Doubling time:
about 24 hours
Harvest:
cell harvest of about 0.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Trichoplusia ni species.
Viruses:
PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list