SPC-BM-36 - Discontinued
ACC 186
Cell line:
SPC-BM-36 - Discontinued
DSMZ no.:
ACC 186
Species:
insect - fall armyworm (Spodoptera frugiperda) (Spodoptera frugiperda)
Cell type:
larval cells
Origin:
Caution: COI DNA barcoding at the DSMZ revealed SPC-BM-36 as originating from a fall armyworm (Spodoptera frugiperda). Cell line SPC-BM-36 must not be used as model for insect Bombyx mori.
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
large, mostly adherent cells; 90% round cells (singly or aggregates), 10% spindled-shaped cells with long processes
Medium:
80% TC 100 medium + 20% h.i. FBS
Subculture:
cells are difficult to culture and grow slowly! split confluent culture 1:3 to 1:6 every 3-4 days using a (plastic) cell scraper
Incubation:
cells grow without CO2 at room temperature (20-25 °C)
Doubling time:
ca. 50-100 hours
Harvest:
cell harvest of up to 12 x 106 cells/80 cm2 flask
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Spodoptera frugiperda species.
Supplied as: