PAB-100
ACC 188
Cell line:
PAB-100
DSMZ no.:
ACC 188
Species:
murine (mouse) (Mus musculus)
Cell type:
hybridoma (anti-SV40 large T viral antigen)
Origin:
established by fusion of NS-1 myeloma cells and spleen cells from a female BALB/c mouse immunized with SVT-2, an SV40-transformed BALB/c mouse cell line; cells reportedly secrete an IgG1 antibody recognizing denaturation-sensitive antigenic determinants unique to large T antigen
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
80% DMEM + 20% h.i. FBS + non-essential amino acids
Subculture:
optimal split ratio 1:2 every 2-3 days; maximum density of about 1.0 x 106 cells/ml should not be exceeded
Incubation:
at 37 °C with 5% CO2
Harvest:
cell harvest of about 0.5-1.0 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Cytogenetics:
predominantly mouse chromosomal morphology with 3 iso-chromosome markers present in most cells - 140(134-155)
Viruses:
ELISA: reverse transcriptase positive
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list