COLO-800
ACC 193
Cell line:
COLO-800
DSMZ no.:
ACC 193
Species:
human (Homo sapiens)
Cell type:
melanoma
Origin:
established from the subcutaneous nodule of a 14-year-old boy with melanoma in 1990
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
90-95% RPMI 1640 + 5-10% h.i. FBS
Subculture:
split confluent culture 1:4 to 1:5 two to three times per week using trypsin/EDTA; seed out at ca. 1-2 x 106 cells/80 cm2
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 48 hours (with 5% FBS)
Harvest:
cell harvest of about 5 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypotriploid karyotype with minor hypertriploid clone and 8% polyploidy - 64(50-69)<3n>X, -Y, -X/Y, -4, -5, +7, -8, -9, -10, -11, -12, +13, +13, +14, +15, -16, -19, +20, -21, -21, der(3)t(3;?)(p11;?), der(13q21q)x2, del(14)(?q11-12q24.2)x2, der(15)t(15;?)(q26;?)x2 - identity confirmed by marker correspondence
Molec. Genetics:
Genomic sequencing and/or ARMS assay show that the cell line carries the BRAF V600E mutation (mu/wt), but no NRAS Q61 mutation
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list