LF-CL2A
ACC 195
Cell line:
LF-CL2A
DSMZ no.:
ACC 195
Species:
rat (Rattus norvegicus)
Cell type:
hepatocarcinoma
Origin:
a primary liver carcinoma was induced by dimethyl-aminoazobenzene (DAB) in a Wistar-Commentry rat; the hepatoma was transferred to a syngeneic rat and a cell line was established and subcloned in 1966
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90-95% DMEM + 5-10% h.i. FBS
Subculture:
split confluent culture about every 2 days using trypsin/EDTA; cells grow quickly; seed about 1 x 106 cells into a 80 cm2 flask
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 20-25 hours
Harvest:
cell harvest of about 30 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Rattus norvegicus species.
Cytogenetics:
rat flat-moded near-triploid karyotype - 58-67<3n> - large submetacentric marker present in most cells
Viruses:
ELISA: reverse transcriptase positive; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list