COLO-704 - Discontinued
ACC 198
Cell line:
COLO-704 - Discontinued
DSMZ no.:
ACC 198
Species:
human (Homo sapiens)
Cell type:
endometrium (cell line COLO-684)
Origin:
Caution: STR analysis demonstrated that cell line COLO-704 is genetically identical to human endometrium carcinoma cell line COLO-684. Cell line COLO-704 must not be used as model for ovarian adenocarcinoma.
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
grows as clusters and as single cells in suspension
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
maintain at 0.5-1.0 x 106 cells/ml; optimal split ratio of 1:2 to 1:3 every 2-3 days; seed out at ca. 0.2-0.3 x 106 cells/ml
Incubation:
5% CO2 (or may be grown in closed flasks), 37 °C
Doubling time:
ca. 30-40 hours
Harvest:
saturation density at about 2.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in the STR profile of cell line COLO-684.
Cytogenetics:
human hyperdiploid karyotype with 22% polyploidy - 51(48-53)<2n>XX, +1, +7, +8, +8, -17, +19, +20, i(1q), der(8)t(8;?)(q21;?) - dm present in 16% cells - i(1q) is associated with ovarian carcinoma - identification confirmed
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as: