human (Homo sapiens)
established from the supraorbital metastasis of a neuroblastoma of a 14-year-old girl in 1971 (see ATCC HTB 10); although described in the original paper as neuroblastoma, SK-N-MC is now widely regarded as having originated from the morphologically similar Askin's tumor related to Ewing's sarcoma; FLI1-EWS gene fusion, the molecular pathological change in Ewing's sarcoma and Askin's tumor, was shown in SK-N-MC by Dunn et al.
Permissions and restrictions:
DSMZ Cell Culture Data:
90% Dulbecco's MEM + 10% h.i. FBS + 2 mM L-glutamine + 1x MEM non-essential amino acids
split confluent culture 1:3 to 1:6 every 3-5 days using trypsin/EDTA; seed out at ca. 1-4 x 106 cells/80 cm2; cells may grow slowly initially
at 37 °C with 10% CO2
ca. 48 hours
cell harvest of about 25 x 106 cells/80 cm2
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
To inquire about expression of EpCAM and intermediate filaments, contact firstname.lastname@example.org.
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
human pseudodiploid karyotype with 6% polyploidy - 46(40-47)<2n>XX, +8, -16, t(1;4)(p36;p13), t(2;3)(q24;q29), der(3)t(3;3)(p13;q22), del(10)(q25), t(11;22)(q24;q12), t(15;22)(q21;q12), add(17)(p12), add(21)(p12) - dmin in 9% of cells - resembles published karyotype - carries masked 11;22 translocation recurrent in Ewing's and Askin's tumor
expression of fusion gene EWS-FLI (type 1) confirmed by RT-PCR
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
|Frozen culture||400,- €|
|Growing culture |
(please inquire for exact delivery time)
|DNA isolated from cell line (25 µg)||500,- €|
|DNA isolated from cell line (5 µg)||120,- €|
see price list