COLO-206F
ACC 21
Cell line:
COLO-206F
DSMZ no.:
ACC 21
Species:
human (Homo sapiens)
Cell type:
colon carcinoma
Origin:
established from the ascites fluid of a 71-year-old man with colon carcinoma in 1975; The cell line was established from the same patient as the cell lines COLO-201 and COLO-205
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
mostly round cells; about 80% are adherent cells; about 2-8% spindle-shaped cells; some giant cells; image
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 1-3 x 106 cells/80 cm2; split confluent cultures about 1:6 once a week using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 2-3 days
Harvest:
about 7-10 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hyperdiploid karyotype with 10% polyploidy - 72(66-77)<2n>XX, -Y, +1, +3, +3, +5, -6, +7, -8, +9, -10, -13, -18, +20, -21, +3mar, i(1q), der(1)t(1;18)(q11;p11), der(3) t(3;?)(p21;?)x2, del(10)(q23.3), der(12)t(3;12)(q11;p13) - a small supernumerary centric fragment or ring present in 18% of cells
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list