BF-F3
ACC 212
Cell line:
BF-F3
DSMZ no.:
ACC 212
Species:
murine (mouse) (Mus musculus)
Cell type:
hybridoma (anti-rat fiber/myosin)
Origin:
this hybridoma cell line was described as secreting a mouse monoclonal antibody (IgM) reacting with adult rat type 2B fibers in immunofluorescence and with rat type 2B MHCs (myosin heavy chain) in Western blotting; established in 1983 by fusion of NSO myeloma cells with spleen cells from a BALB/c mouse immunized with purified myosin from fetal bovine muscles
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
90% DMEM (4.5 g/L glucose) + 10% h.i. FBS + 2 mM L-glutamine
Subculture:
seed out at ca. 0.2 x 106 cells/ml; maintain at 0.2-0.5 x 106 cells/ml; optimal split ratio of 1:2 to 1:4 every 2-4 days
Incubation:
at 37 °C with 10% CO2
Doubling time:
20-30 hours
Harvest:
maximum density at about 1.0 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
secretion of a IgM monoclonal antibody
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Cytogenetics:
murine chromosome morphology
Viruses:
ELISA: reverse transcriptase negative; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list