A4-951
ACC 214
Cell line:
A4-951
DSMZ no.:
ACC 214
Species:
murine (mouse) (Mus musculus)
Cell type:
hybridoma (anti-fiber/myosin)
Origin:
described as secreting a mouse monoclonal antibody (IgG1) reacting with adult slow fibers in rat and human and with slow myosin heavy chain on Western blots; the cell line was established in 1985 by fusion of X63AG8.653 cells with spleen cells from a BALB/c mouse that had been immunized with human skeletal muscle myosin
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% DMEM (4.5 g/L glucose) + 10% h.i. FBS + 2 mM L-glutamine + 1 mM sodium pyruvate + 1x non-essential amino acids
Subculture:
split saturated culture 1:2 to 1:3 every 2-3 days; seed out at ca. 0.2 x 106 cells/ml; optimal density of about 0.2 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 24 hours
Harvest:
maximal density of about 0.8 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization assays
Immunology:
secretion of a IgG1 monoclonal antibody
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Cytogenetics:
murine flat-moded hypertetraploid karyotype - 81(42-92)<4n> - 1-2 dmin and 1-2 centric fusion markers present in most cells
Viruses:
ELISA: reverse transcriptase negative; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list