Species:
human (Homo sapiens)
Cell type:
B cell lymphoma
Origin:
established in 1998 from the pleural effusion of a 35-year-old man with primary effusion lymphoma (PEL) and a history of Kaposi's sarcoma; cells are HHV-8-positive, but HIV-negative. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and
RNA-Seq)
Risk assessment:
The cell line carries HHV-8 sequences which were detected by PCR and Southern blot. We did not determine the infection mode of this herpes virus and cannot exclude the secretion of the viruses. The cell line is thus categorized biosafety level 2.
Permissions and restrictions:
Morphology:
single round to pleomorph cells growing in suspension;
image;
image
Medium:
80-85% RPMI 1640 + 15-20% h.i. FBS
Subculture:
split saturated culture 1:3 every 3-4 days; maintain at ca. 0.5-1.0 x 106 cells/ml; seed out at ca. 1.0 x 106 cells/ml in 24- or 12-well plates; viability may be low after thawing and during the first 1-2 weeks
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 35-40 hours
Harvest:
maximal density at ca. 1.4 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
CD3 -, CD10 -, CD13 -, CD19 -, CD34 -, CD38 +, cyCD79a -, CD80 -, CD138 -, HLA-DR +, sm/cy IgG -, sm/cy IgM -, sm/cy kappa -, cm/cy lambda -;
image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hyperdiploid karyotype with 12% polyploidy - 44-49<2n>X, -Y, +8, +2mar, del(4)(q25), add(8)(p11), dup(12)(q11q14), del(14)(q24) - sideline with i(8q) - resembles published karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HHV-8 +, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | | Prices |
|
| Frozen culture | | 450,- € |
Growing culture
(please inquire for exact delivery time) | | 900,- € |
| DNA isolated from cell line (25 µg) | | 560,- € |
| DNA isolated from cell line (5 µg) | | 140,- € |
see price list
