SC-71
ACC 217
Cell line:
SC-71
DSMZ no.:
ACC 217
Species:
murine (mouse) (Mus musculus)
Cell type:
hybridoma (anti-rat fiber/myosin)
Origin:
described as secreting a mouse monoclonal antibody (IgG1) reacting with adult rat skeletal muscle type 2A (but not 2B) fibers in immunofluorescence and with undigested type 2A MHCs (myosin heavy chain) as well as both rod and light meromyosin fragments in Western blotting; rat neonatal and embryonic MHCs were not studied by Western blotting; established in 1985 by fusion of NSO myeloma cells with spleen cells from a BALB/c mouse immunized with purified myosin from bovine subcutaneous muscle
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% DMEM (4.5 g/L glucose) + 10% h.i. FBS + 2 mM L-glutamine
Subculture:
maintain at 0.1-0.5 x 106 cells/ml; split saturated culture at 1:5 to 1:10 every 2-5 days; seed out at ca. 0.5 x 106 cells/ml; use initially 24-well plate culture dishes and 20% FBS; daily exchange of medium is recommended
Incubation:
at 37 °C with 7.5% CO2
Doubling time:
ca. 20 hours
Harvest:
maximal density at about 1.0 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
secretion of a IgG1 monoclonal antibody
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Cytogenetics:
murine karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list