LCL-WEI
ACC 218
Cell line:
LCL-WEI
DSMZ no.:
ACC 218
Species:
human (Homo sapiens)
Cell type:
B lymphoblastoid cells (from patient with melanoma)
Origin:
Epstein-Barr virus-positive cell line established from peripheral blood in 1977 from male patient with melanoma
Reference(s):
Biosafety level:
1
Risk assessment:
The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were negative by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1. According to a recommendation of the German Central Committee for Biological Safety (ZKBS) the cells have to be handled under biosafety level 2 when the cells are used in genetic engineering projects.
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
80-90% RPMI 1640 + 10-20% h.i. FBS
Subculture:
maintain at about 0.3-1.0 x 106 cells/ml; optimal split ratio of 1:3 to 1:8 every 2-4 days; the culture usually contains a large amount of cell debris
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 30-40 hours
Harvest:
saturation density at about 2.0 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
initial contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization assays
Immunology:
CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD80 +, HLA-DR +, cyIgG +, cyIgM -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human near-tetraploid karyotype with diploid (36%) sideline and 4% polyploidy - 90(88-94)<4n>XX/XXY/XXYY - no consistent structural aberrations
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list