BF-45
ACC 221
Cell line:
BF-45
DSMZ no.:
ACC 221
Species:
murine (mouse) (Mus musculus)
Cell type:
hybridoma (anti-rat fiber/myosin)
Origin:
described as secreting a mouse monoclonal antibody (IgG1) that reacts with rat embryonic fibers in immunofluorescence and with embryonic myosin heavy chains in Western blotting; established by fusion of NSO myeloma cells with spleen cells from a BALB/c mouse immunized with purified myosin from fetal bovine muscles
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
90% DMEM (4.5 g/L glucose) + 10% horse serum + 2 mM L-glutamine
Subculture:
seed out at ca. 0.25 x 106 cells/ml; split saturated culture at 1:3 to 1:4 every 2 days; viability may be low after thawing, but cells will recover soon
Incubation:
at 37 °C with 10% CO2
Doubling time:
ca. 45 hours
Harvest:
maximal density at about 1.0 x 106 cells/ml
Storage:
frozen with 70% medium, 20% horse serum, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
secretion of a IgG1 monoclonal antibody
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Cytogenetics:
murine flat-moded hypertetraploid karyotype with 5% polyploidy - 78-93<4n> - 1-2 dmin present in most cells
Viruses:
ELISA: reverse transcriptase negative; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list