G/G
ACC 224
Cell line:
G/G
DSMZ no.:
ACC 224
Species:
murine (mouse) (Mus musculus)
Cell type:
urinary bladder
Origin:
established from an adult female C3H/E1 mouse; non-tumorigenic urine bladder cell line immortalized in vitro
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
45% Ham's F10 + 45% DMEM + 10% h.i. FBS + 5 µg/ml human insulin + 5 µg/ml human transferrin + 50 nM hydrocortisone + 5 ng/ml selenite
Subculture:
seed out at ca. 2 x 106 cells/80 cm2; split confluent culture 1:3 to 1:10 every 3-7 days using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 35-50 hours
Harvest:
harvest of about 0.5 x 105 cells/cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Cytogenetics:
murine near-tetraploid karyotype with 3.6% polyploidy - 80/81(76-83)<4n> - up to 3 (sub)metacentric markers present in most cells arising by centric fusion
Viruses:
ELISA: reverse transcriptase negative; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list