NUC-5
ACC 226
Cell line:
NUC-5
DSMZ no.:
ACC 226
Species:
murine (mouse) (Mus musculus)
Cell type:
urinary bladder
Origin:
established from an adult female C3H/Law mouse; non-tumorigenic in vitro immortalized urine bladder cell line with aberrant growth factor response, aneuploid (chromosome number 70-75)
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
45% Ham's F10 + 45% DMEM + 10% h.i. FBS + 5 µg/ml human insulin + 5 µg/ml human transferrin + 50 nM hydrocortisone + 5 ng/ml selenite + 10 µM HEPES
Subculture:
split confluent culture 1:4 every 6-7 days using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 60-75 hours
Harvest:
harvest of 2-3 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Cytogenetics:
murine hypotetraploid karyotype with 14% polyploidy and 1-3 dmin in 34% cells - 70(64-74)<4n> - 1-3 centric fusion markers present - resembles published karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Growing culture (please inquire for exact delivery time) | 1040,- € | |
| Frozen culture | 520,- € | |
| DNA isolated from cell line (25 µg) | 650,- € | |
| DNA isolated from cell line (5 µg) | 160,- € | |
see price list