SBC-2 - Discontinued

human (Homo sapiens)

cervix carcinoma (cell line HELA)

Caution: Cytogenetic and STR analyses demonstrated that cell line SBC-2 is genetically identical to HELA. SBC-2 cells must not be used to model urinary bladder carcinoma.

14396, 15014

1

The cell line is infected with papilloma virus type 18 (HPV-18). The cells contain several copies as proviruses integrated into the eukaryotic genome. The integrated virus genomes are incomplete and exhibit 2-3 kb deletions of the E2-L2 region. An activation and transmission of the HPV-18 during handling of the cell line is improbable. The cell line is categorized biosafety level 1.

A

epitheloid cells growing adherently in monolayers, often triangle-formed cells

90% RPMI 1640 + 10% h.i. FBS

split confluent culture 1:10 to 1:20 every second day using trypsin/EDTA; seed out at about 1 x 10⁶ cells/175 cm²

at 37 °C with 5% CO₂

about 40 hours

cell harvest of about 20-25 x 10⁶ cells/175 cm²

frozen with 70% medium, 20% FBS, 10% DMSO

contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization assays

To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in the STR profile of cell line HELA.

human hypertriploid karyotype with 2% polyploidy - 75(66-80)<3n>XX, -X, +1, +3, -4, +5, +6, +8, -9, +10, +11, +11, +12, -13, -14, -15, +16, +17, -18, -22, +3-5mar, del(3)(q11), der(3)t(1;3)(q11;q13), der(3)t(3;5)(q13;q11), i(5p), i(7p)x1-2, der(8)t(8;11)(q24;q11), der(9)t(9;11)(p21;q11), i(11p)x3, add(12)(?q21), add(15)(p11), del(17)(p12) - karyotype closely related to SBC-7 - HELA markers present

ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HPV +, HTLV-1/2 -, MLV -, SMRV -

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