SC-75
ACC 232
Cell line:
SC-75
DSMZ no.:
ACC 232
Species:
murine (mouse) (Mus musculus)
Cell type:
hybridoma (anti-rat fiber/myosin)
Origin:
described as secreting a mouse monoclonal antibody (IgM) reacting with all adult rat type 2 fibers in immunofluorescence and with all adult rat type 2 MHCs (myosin heavy chain) in Western blotting; recognizing also rat neonatal fibers in immunofluorescence and rat neonatal MHCs in Western blotting; established in 1985 by fusion of NSO myeloma cells with spleen cells from a BALB/c mouse immunized with purified myosin from bovine subcutaneous muscle
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
90% DMEM (4.5 g/L glucose) + 10% horse serum + 2 mM L-glutamine + HFCS ("Hybridoma Fusion and Cloning Supplement") for the first 1 week post-thawing
Subculture:
maintain at 0.5-1.0 x 106 cells/ml; split saturated culture at 1:2 to 1:3 every 2 day (maximal split ratio of 1:5); viability may drop below 50% after thawing; we recommend to start culture with a cell concentration of ca. 0.5 x 106 cells/ml and to cultivate initially in 24-well plates with medium containing 20% horse serum
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 23 hours
Harvest:
maximal density at about 2.0 x 106 cells/ml
Storage:
frozen with 70% medium, 20% horse serum, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
secretion of a IgM monoclonal antibody
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Cytogenetics:
murine hypertetraploid karyotype with 5% polyploidy - 86-96<4n> - 1-3 centric fusion markers and 1-2 dmin present in most cells
Viruses:
ELISA: reverse transcriptase negative; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list