IGR-39
ACC 239
Cell line:
IGR-39
DSMZ no.:
ACC 239
Species:
human (Homo sapiens)
Cell type:
melanoma
Origin:
established from the primary (achromic) cutaneous tumor (left thigh) of a 26-year-old man with malignant melanoma (primary tumor histology: SSM level IV); a cell line IGR-39 from a lymph node metastasis is available (DSMZ ACC 237); described as tumorigenic in nude mice
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
85% DMEM + 15% h.i. FBS
Subculture:
seed out at ca. 1 x 106 cells/80 cm2; split subconfluent or confluent culture 1:4 every 3-4 days using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 30 hours
Harvest:
maximal density of about 6-8 x 106/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database matching the profile of sister cell line IGR-37
Cytogenetics:
human flat-moded tetra-/pentaploid karyotype - 104-112<4n>XXY - rearrangements include der(1)t(1;?21)(p13;q11), der(16)t(1;16)(p13;q23) also present in the autologous sister line IGR-37
Molec. Genetics:
Genomic sequencing and/or ARMS assay show that the cell line carries the BRAF V600E mutation (mu/wt), but no NRAS Q61 mutation
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list