WEHI-164S
ACC 25
Cell line:
WEHI-164S
DSMZ no.:
ACC 25
Species:
murine (mouse) (Mus musculus)
Cell type:
fibrosarcoma
Origin:
established from a fibrosarcoma chemically induced in a BALB/c mouse; cells were described in the literature to be sensitive to human cytotoxic monocytes, to tumor necrosis factor (TNF-alpha) and lymphotoxin (TNF-beta)
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
split confluent cultures at 1:3 to 1:6; cells are adherent, slapping of the back of the flask by hand will detach the cells; seed out at ca. 4 x 106 cells/80 cm2 in 10 ml medium
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 30-50 hours
Harvest:
ca. 15 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Cytogenetics:
murine hypertriploid karyotype with 20% polyploidy - 60-62<3n> - supernumerary ring/acf but no centric fusion marker present in most cells
Viruses:
ELISA: reverse transcriptase negative; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list