RGE
ACC 262
Cell line:
RGE
DSMZ no.:
ACC 262
Species:
rat (Rattus norvegicus)
Cell type:
endothelial glomerular kidney
Origin:
established from freshly isolated glomeruli from kidneys of 6-week-old female Sprague-Dawley rats
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
split confluent culture 1:2 to 1:3 twice a week using trypsin/EDTA (cells are only lightly adherent and can be detached by tapping the flask; however, to prepare single cell suspension, trypsin/EDTA treatment is necessary); seed out at ca. 2 x 106 cells/80 cm2
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 17 hours
Harvest:
cell harvest of about 40-50 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Rattus norvegicus species.
Cytogenetics:
rat hypertriploid karyotype with 6% polyploidy - 67(67-72)<3n> - corresponds to published karyotype
Viruses:
ELISA: reverse transcriptase positive; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list