TE-671 - Discontinued

human (Homo sapiens)

rhabdomyosarcoma (cell line RD)

Caution: STR analysis established that cell line TE-671 is genetically identical to human rhabdomyosarcoma cell line RD. Cell line TE-671 must not be used as model for medulloblastoma.

1

A

round and elongated cells with spindle-shape extensions; the round cells contain almost no cytoplasm; cells grow adherent as monolayer

80-85% DMEM + 15-20% h.i. FBS

seed out about 1 x 10⁶ cells/80 cm²; split culture before cells are entirely confluent, (1:3 to 1:5), using trypsin/EDTA

at 37 °C with 10% CO₂

70-80 hours

cell harvest of about 4 x 10⁶ cells/175 cm²

frozen with 70% medium, 20% FBS, 10% DMSO

negative in DAPI, microbiological culture, RNA hybridization, PCR assays

To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in the STR profile of cell line RD.

human bimodal hyperdiploid/tetraploid karyotype with 9.7% polyploidy - 43-50/86-98<4n>XX, -X, -X, +1, -2, -4, +7, +8, -9, -9, -13, -14, -14, -15, -15,- 16, -16, -17, -18, -18, -19, -19, -20, -20, -21, -21, +8-11mar, del(1)(p31), add(1)(p31), i(2p), add(3)(p11), add(5)(q35)x2, del(6)(q22)x2, add(9)(q34)x2, add(12)(q24)x2, der(13)t(6;13)(p12.1;p13)x2, add(14)(p11), add(?14)(q24), i(17q), der(19)t(17;19)(q12;p/q13)x2 - ploidy resembles RD embryonal rhabdomyosarcoma of which TE-671 is a subline

ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -

see price list

Print data sheet