DMBM-2
ACC 269
Cell line:
DMBM-2
DSMZ no.:
ACC 269
Species:
murine (mouse) (Mus musculus)
Cell type:
macrophages
Origin:
established from the bone marrow of a C3H/HeJ mouse
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
80% RPMI 1640 + 20% horse serum + 1 mM sodium pyruvate + 1 mM non-essential amino acids + 1.25 µg/ml vitamin B12 + 75 µM alpha-thioglycerin + 2 mM L-glutamine + 2.5% 10x L-929 supernatant (DSM ACC 2)
Subculture:
split 1:10 every 4-6 days detaching the cells by flushing the adherent cells with medium through a 25G needle (do not try to desaggregate cells by passing through the needle); cells grow best in Corning round plates (100 x 20 mm)
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 50 hours
Harvest:
cell harvest of about 3-5 x 106 cells/plate
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Cytogenetics:
murine near-diploid karyotype with 10% tetraploidy - 42(37-43)<2n> - centric fusion markers absent
Viruses:
ELISA: reverse transcriptase negative; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list