MUTZ-2

human (Homo sapiens)

acute myeloid leukemia

established from the peripheral blood of a 62-year-old man with acute myeloid leukemia (AML FAB M2) at diagnosis in 1993; cells are growth factor-dependent and responsive to IL-3, SCF, G-CSF, M-CSF and IFN-gamma

14482, 14618

1

A

Transfer to third parties needs to be negotiated with the depositor.

single cells in suspension, growing partly in clumps ; image

60% alpha-MEM (with ribo- and deoxyribonucleosides) + 20% h.i. FBS + 20% vol conditioned medium of cell line 5637 (DSM ACC 35) (cells grow well with 50 ng/ml SCF)

seed out at about 0.5 x 10⁶ cells/ml; cells should be grown in 24-well-plates; optimal split ratio of 1:2 to 1:3 twice a week; CAVEAT: by favoring selective growth, suboptimal culture of cytokine-dependent cell lines may promote outgrowth of factor-independent subclones. Selective conditions include cytokine insufficiency and inadequate cell density. Thus the DSMZ cannot guarantee indefinite stability of factor-dependence of cell lines.

at 37 °C with 5% CO₂

about 47 hours

maximum density at about 1.5-2.0 x 10⁶ cells/ml

frozen with 70% medium, 20% FBS, 10% DMSO

negative in DAPI, microbiological culture, RNA hybridization, PCR assays

CD3 -, CD4 +, CD5 -, CD7 -, CD8 -, CD10 -, CD13 +, CD14 -, CD15 +, CD19 -, CD20 -, CD33 +, CD34 +, CD71 +, CD235a -, HLA-DR +; image

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.

human hyperdiploid karyotype with 1.8% polyploidy - 48(46-50)<2n>XY, +8, +10 - no structural aberrations detected - +8 is a typical primary change in AML M1

ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -

Delivery form		Prices
Frozen culture		450,- €
Growing culture   (please inquire for exact delivery time)		900,- €
DNA isolated from cell line (25 µg)		560,- €
DNA isolated from cell line (5 µg)		140,- €

see price list

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