MUTZ-2
ACC 271
Cell line:
MUTZ-2
DSMZ no.:
ACC 271
Species:
human (Homo sapiens)
Cell type:
acute myeloid leukemia
Origin:
established from the peripheral blood of a 62-year-old man with acute myeloid leukemia (AML FAB M2) at diagnosis in 1993; cells are growth factor-dependent and responsive to IL-3, SCF, G-CSF, M-CSF and IFN-gamma
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Morphology:
single cells in suspension, growing partly in clumps; image
Medium:
60% alpha-MEM (with ribo- and deoxyribonucleosides) + 20% h.i. FBS + 20% vol conditioned medium of cell line 5637 (DSM ACC 35) (cells grow well with 50 ng/ml SCF)
Subculture:
seed out at about 0.5 x 106 cells/ml; cells should be grown in 24-well-plates; optimal split ratio of 1:2 to 1:3 twice a week; CAVEAT: by favoring selective growth, suboptimal culture of cytokine-dependent cell lines may promote outgrowth of factor-independent subclones. Selective conditions include cytokine insufficiency and inadequate cell density. Thus the DSMZ cannot guarantee indefinite stability of factor-dependence of cell lines.
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 47 hours
Harvest:
maximum density at about 1.5-2.0 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
CD3 -, CD4 +, CD5 -, CD7 -, CD8 -, CD10 -, CD13 +, CD14 -, CD15 +, CD19 -, CD20 -, CD33 +, CD34 +, CD71 +, CD235a -, HLA-DR +; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hyperdiploid karyotype with 1.8% polyploidy - 48(46-50)<2n>XY, +8, +10 - no structural aberrations detected - +8 is a typical primary change in AML M1
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list