CRO-AP3
ACC 275
Cell line:
CRO-AP3
DSMZ no.:
ACC 275
Species:
human (Homo sapiens)
Cell type:
B cell lymphoma
Origin:
established from the malignant primary effusion lymphoma (PEL) of a 42-year-old HIV+ man at diagnosis (prior to therapy) with a previous history of Kaposi's sarcoma; cells were shown to carry the human herpesvirus 8 (HHV-8), but not the Epstein-Barr virus (EBV) and not the human immunodeficiency virus (HIV)
Biosafety level:
2
Risk assessment:
The cell line carries HHV-8 sequences which were detected by PCR and Southern blot. We did not determine the infection mode of this herpes virus and cannot exclude the secretion of the viruses. The cell line is thus categorized biosafety level 2.
DSMZ Cell Culture Data:
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
split saturated culture 1:2 to 1:3 every second day; seed out at ca. 0.3 x 106 cells/ml; maintain at ca. 0.2-0.3 x 106 cells/ml; maximal density at ca. 0.6 x 106 cells/ml (maximal density should not be exceeded); initially after thawing, the viability may drop to as low as 30-40% for about 1-2 weeks
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50 hours
Harvest:
cell harvest of ca. 0.4 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
CD4 -, CD10 -, CD13 -, CD19 -, CD20 -, CD34 -, CD37 -, cyCD79a -, CD80 -, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded hypotetraploid karyotype - 80-88<4n>XX, -Y, -Y, -1, -2, +6, -7, -10, -13, -13, -22, -22, +6mar, i(1q), add(2)(p2?), add(3)(q2?7), der(4;9)(q10;q10)x1-2, del(5)(p13p15)x1-2, del(5)(q2?1)x1-2, der(6)t(6;7)(q15;q11)add(7)(q3?5), del(8)(p11), del(9)(p21), add(9)(p11), del(11)(q2?2q2?3)x2, der(12)t(2;12)(p12;q24.32), add(13)(p11), t(14;19)(q11;q13)x2, der(15)t(1;?)(?15)(q21;)(?;q11), ider(?)t(8;?)(q11;?) - extensive subclonal rearrangements present - matches published karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 +, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list