4H1-A7
ACC 28
Cell line:
4H1-A7
DSMZ no.:
ACC 28
Species:
murine (mouse) (Mus musculus)
Cell type:
hybridoma (anti-canine adenovirus 2)
Origin:
established by fusion of SP2/0-AG14 myeloma cells with BALB/c mouse spleen cells immunized with purified canine adenovirus type 2 (CAV-2, Manhattan strain); the secreted antibody (IgG2a) binds to and neutralizes CAV-2 and is type-specific
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
92.5% DMEM (4.5 g/L glucose) + 5% horse serum + 2.5% h.i. FBS
Subculture:
maintain at 0.5-1.0 x 106 cells/ml; optimal split ratio of 1:2 every 2-3 days
Incubation:
at 37 °C with 5% CO2
Harvest:
maximum density at 1.0-1.5 x 106 cells/ml
Storage:
frozen with 75% medium, 10% horse serum, 5% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Cytogenetics:
murine hypertetraploid/pentaploid karyotype with 5% polyploidy - 91-98<4n> - 3 centric fusion markers and 1-3 dmin present in most cells
Viruses:
ELISA: reverse transcriptase negative; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list