PAB-1620
ACC 280
Cell line:
PAB-1620
DSMZ no.:
ACC 280
Species:
murine (mouse) (Mus musculus)
Cell type:
hybridoma (anti-wild type p53)
Origin:
established in 1982 by immunizing BALB/c mice with VLM tumor cells and fusing splenocytes with SP2/0-Ag14 myeloma cells; described as secreting a mouse monoclonal antibody (IgG2akappa) detecting epitopes of wild type p53 (not mutant or denatured p53), that occur on human, primate, bovine, mouse and rat cells, by ELISA and immunoblotting
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% DMEM + 10% h.i. FBS + 4 mM L-glutamine
Subculture:
split saturated culture optimally 1:2 to 1:3 every second day; seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.3-0.4 x 106 cells/ml; maximal density at ca. 0.7 x 106 cells/ml; some cells attach to the flask surface, but may be detached by tapping the flask; if viability decreases, addition of HFCS ("Hybridoma Fusion and Cloning Supplement") may be useful
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 24 hours
Harvest:
cell harvest of ca. 0.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
secretion of IgG2a monoclonal antibody
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Cytogenetics:
murine flat-moded hypertetraploid karyotype - 82-97<4n> - several centric fusion markers present
Viruses:
ELISA: reverse transcriptase positive
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list