TI-4
ACC 286
Cell line:
TI-4
DSMZ no.:
ACC 286
Species:
murine (mouse) (Mus musculus)
Cell type:
hybridoma (anti-rat fiber/troponin I)
Origin:
described as secreting a mouse monoclonal antibody (IgG1) reacting with rat cardiac fibers in immunofluorescence and with rat adult and fetal cardiac troponin I in Western blotting; this cell line was produced by fusing NSO myeloma cells with spleen cells from a BALB/c mouse immunized with purified troponin complex from bovine adult heart
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
80% DMEM (4.5 g/L glucose) + 10-20% horse serum + 2 mM L-glutamine
Subculture:
split saturated culture at 1:2 to 1:8 every 3-6 days; after thawing seed out at about 0.8 x 106 cells/ml and culture initially at 20% horse serum in a 24-well-plate
Incubation:
at 37 °C with 10% CO2
Harvest:
maximal density at about 1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% horse serum, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization assays
Immunology:
secretion of a IgG1 monoclonal antibody
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Cytogenetics:
murine karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list