P-388D1(IL-1)
ACC 288
Cell line:
P-388D1(IL-1)
DSMZ no.:
ACC 288
Species:
murine (mouse) (Mus musculus)
Cell type:
monocytes-macrophages
Origin:
established from the ascites of a DBA/2 mouse with a lymphoid neoplasm induced by methylcholanthrene and subsequently enriched for adherent (monocyte-macrophage) cells by selection (yielding this IL1-producing subclone); reported to produce IL-1 after stimulation with LPS or PMA, to phagocytize latex particles and to synthesize lysozyme
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
adherent elliptical and round loosely adherent cells growing in monolayers
Medium:
80-90% RPMI 1640 + 10-20% horse serum + 3.5 g/L glucose
Subculture:
seed out at ca. 1-3 x 106 cells/175cm2 flask; split confluent culture 1:3 to 1:6 every 3-6 days using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 30-60 hours (according to density)
Harvest:
cell harvest of ca. 25 x 106 cells/175cm2 flask
Storage:
frozen with 70% medium, 15% horse serum, 5% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Cytogenetics:
murine hypertriploid karyotype with 8% polyploidy - 61(57-65)<3n> - 5-8 centric fusion markers
Viruses:
ELISA: reverse transcriptase negative; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list