RV-C2
ACC 289
Cell line:
RV-C2
DSMZ no.:
ACC 289
Species:
murine (mouse) (Mus musculus)
Cell type:
hybridoma (anti-rat fiber/troponin)
Origin:
described as secreting a mouse monoclonal antibody (IgG2b) reacting with fetal, neonatal and adult cardiac fibers in immunofluorescence and with rat cardiac fetal and adult troponin T in Western blotting; in immunofluorescence this antibody stains some rat fetal and neonatal skeletal muscle fibers which express fetal cardiac troponin T as also shown by the Western blotting of rat fetal and neonatal skeletal muscle; this cell line was produced by fusing NSO myeloma cells with spleen cells from a Balb/c mouse that had been immunized with a myofibrillar preparation from hypothyroid rabbit ventricle
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
80% DMEM (4.5 g/L glucose) + 20% horse serum + 2 mM L-glutamine
Subculture:
optimal split ratio of 1:2 to 1:3 twice a week; seed out at about 0.5-1.0 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 32 hours
Harvest:
maximal density at about 2.0 x 106 cells/ml
Storage:
frozen with 70% medium, 20% horse serum, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization assays
Immunology:
secretion of a IgG2b monoclonal antibody
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Cytogenetics:
murine hypertetraploid/pentaploid karyotype - 88-99<4n> - 2 centric fusion markers and 1-2 dmin present in most cells
Viruses:
ELISA: reverse transcriptase negative; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list