A4-1025
ACC 296
Cell line:
A4-1025
DSMZ no.:
ACC 296
Species:
murine (mouse) (Mus musculus)
Cell type:
hybridoma (anti-fiber/myosin)
Origin:
described as secreting a mouse monoclonal antibody (IgG2a) detecting all fibers in skeletal muscle and myosin heavy chains on Western blots and detecting mammalian, chicken, zebrafish, axolotl, xenopus skeletal muscle myosin heavy chains; the cell line was established in 1985 by fusion of X63AG8.653 cells with spleen cells from a BALB/c mouse that had been immunized with human skeletal muscle myosin
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
90% DMEM (4.5 g/L glucose) + 10% h.i. FBS + 2 mM L-glutamine + 1 mM sodium pyruvate + 1x non-essential amino acids
Subculture:
split saturated culture 1:5 to 1:10 every 2-3 days; seed out at about 1.0-1.5 x 105 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 40-50 hours
Harvest:
cell harvest of about 0.5-1.0 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization assays
Immunology:
secretion of a IgG2a monoclonal antibody
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Cytogenetics:
murine hypertetraploid karyotype with 5% polyploidy - 84(79-84) - one large submetacentric and up to 5 centric fusion markers present
Viruses:
ELISA: reverse transcriptase positive
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list