A4-1077
ACC 297
Cell line:
A4-1077
DSMZ no.:
ACC 297
Species:
murine (mouse) (Mus musculus)
Cell type:
hybridoma (anti-fiber/myosin)
Origin:
described as secreting a mouse monoclonal antibody (IgG1) reacting with human nascent secondary fibers and adult fast fibers and myosin heavy chains in Western blots (does not detect rat); the cell line was established in 1985 by fusion of X63AG8.653 cells with spleen cells from a BALB/c mouse that had been immunized with human skeletal muscle myosin
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
85% DMEM (4.5 g/L glucose) + 15% h.i. FBS + 2 mM L-glutamine + 1 mM sodium pyruvate + 1x non-essential amino acids
Subculture:
split saturated culture 1:2 to 1:3 every 2 days; optimal density of about 0.5 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 20-40 hours
Harvest:
maximal density of about 0.6-1.0 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization assays
Immunology:
secretion of a IgG1 monoclonal antibody
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Cytogenetics:
murine flat-moded hypertetraploid/pentaploid karyotype with 10% polyploidy - 90(81-96)<4n> - 2-3 centric fusion markers and 1-2 dmin present in most cells
Viruses:
ELISA: reverse transcriptase positive; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list