
CAL-51
ACC 302
Cell line:
CAL-51
DSMZ no.:
ACC 302
Species:
human (Homo sapiens)
Cell type:
breast carcinoma
Origin:
established from the pleural effusion metastasis of a 45-year-old woman with progressive ductal breast adenocarcinoma (after radio-, chemotherapy and surgery) in 1985; rare example of tumor cell line with normal karyotype; described as triple-negative cell line; listed in the CCLE (ref 18232);
RNA sequence data are available (see Ref 40906 and RNA-Seq)
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
80-90% DMEM (4.5 g/L glucose) + 10-20% h.i. FBS
Subculture:
seed out at ca. 1 x 106 cells/80 cm2; split culture 1:2 to 1:4 once or twice a week using trypsin/EDTA before cells reach complete confluency
Incubation:
at 37 °C with 10% CO2
Doubling time:
24-30 hours
Harvest:
cell harvest of ca. 15-20 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human near-diploid karyotype with 28% tetraploidy - 46<2n>XX, no consistent abnormality detected - rare example of tumor cell line with normal karyotype - resembles published karyotype
Molec. Genetics:
triple-negative confirmed by absence of ER, PR and weak HER2 expression in Western blots
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
Delivery form | Prices | |
Frozen culture | 450,- € | |
Growing culture (please inquire for exact delivery time) | 900,- € | |
DNA isolated from cell line (25 µg) | 560,- € | |
DNA isolated from cell line (5 µg) | 140,- € |
see price list