F1-652
ACC 309
Cell line:
F1-652
DSMZ no.:
ACC 309
Species:
murine (mouse) (Mus musculus)
Cell type:
hybridoma (anti-fiber/myosin)
Origin:
described as secreting a mouse monoclonal antibody (IgG1) reacting with embryonic fibers in rodent and human muscle and with embryonic myosin heavy chain on Western blots; the cell line was established in 1985 by fusion of X63AG8.653 cells with spleen cells from a BALB/c mouse that had been immunized with human skeletal muscle myosin
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% DMEM (4.5 g/L glucose) + 10% h.i. FBS + 2 mM L-glutamine + 1 mM sodium pyruvate + 1x non-essential amino acids
Subculture:
split saturated culture 1:3 to 1:5 once or twice a week; cultivate optimally between 0.1-1.0 x 106 cells/ml
Incubation:
at 37 °C with 10% CO2
Doubling time:
about 30-36 hours
Harvest:
maximal density of about 2.0 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization assays
Immunology:
secretion of a IgG1 monoclonal antibody
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Cytogenetics:
murine hypertetraploid karyotype - 87(72-90)<4n> - carries 2-3 centric fusion markers and 1-2 dm
Viruses:
ELISA: reverse transcriptase positive
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list