Leibniz Institute DSMZ: Details

Cell line:

SW-480

DSMZ no.:

ACC 313

Species:

human (Homo sapiens)

Cell type:

colon adenocarcinoma

Origin:

established from the tumor of a 50-year-old man with colon adenocarcinoma (grade 4, Duke class B)

Reference(s):

14713

Biosafety level:

1

Permissions and restrictions:

A

DSMZ Cell Culture Data:

Morphology:

endothelial-like adherent cells, mostly bipolar with microvilli growing in monolayers; image; image; image; image; image

Medium:

90-95% RPMI 1640 + 5-10% h.i. FBS

Subculture:

seed out at about 0.5-1.0 x 10⁶ cells/80 cm²; split confluent culture 1:5 to 1:10 once or twice a week using trypsin/EDTA

Incubation:

at 37 °C with 5% CO₂

Doubling time:

25-30 hours

Harvest:

cell harvest of about 10-15 x 10⁶ cells/80 cm²

Storage:

frozen with 70% medium, 20% FBS, 10% DMSO

DSMZ Scientific Data:

Mycoplasma:

negative in DAPI, microbiological culture, RNA hybridization, PCR assays

Immunology:

To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.

Authenticity:

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.

Cytogenetics:

human hypotriploid karyotype with 12% polyploidy - 58(51-58)<3n>XX, -Y, -1, -3, -4, -5, -6, +7, -8, -10, -12, -15, -18, -19, +20, -22, der(1)t(1;9)(q22;q12), t(2;12)(q35;q13.1), del(5)(q13.2q32), add(7)(q35), dup(7)(q11.22q21.3), der(8)t(1;8)(p11;p11)t(1;9)(q22;q12), add(10)(p14), inv(14)(q13q32), del(18)(q12.2), der(19)t(6;19)(p11;q13), der(20)t(14;20)(q13;p13)x2 - identified by large submetacentric der(2)t(2;12) marker

Viruses:

ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -

Supplied as:

Delivery form		Prices

Frozen culture		450,- €
Growing culture (please inquire for exact delivery time)		900,- €
DNA isolated from cell line (25 µg)		560,- €
DNA isolated from cell line (5 µg)		140,- €

see price list

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