KPL-1 (MCF-7)
ACC 317
Cell line:
KPL-1 (MCF-7)
DSMZ no.:
ACC 317
Species:
human (Homo sapiens)
Cell type:
breast carcinoma
Origin:
this cell line is a subclone of MCF-7 (DSMZ ACC 115) with comparable but not identical properties; differences in expression of MUC-1 and the androgen receptor were described
; RNA sequence data are available (see Ref 40906 and RNA-Seq)
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% DMEM + 10% h.i. FBS
Subculture:
seed out at ca. 1-2 x 106 cells/80 cm2; split confluent culture 1:4 to 1:8 once or twice a week using trypsin/EDTA
Incubation:
at 37 °C with 10% CO2
Doubling time:
ca. 50 hours
Harvest:
cell harvest of ca. 5 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database, identical to that of cell line MCF-7
Cytogenetics:
human highly rearranged hypertriploid karyotype with 4% polyploidy - 77(70-82)<3n>XX, -X, -1, +2, -11, -13, +14, +15, -17, -18, -19, -20, -20, -21, -22, +15mar - add(X)(q28), add(2)(q35), add(3)(p25)x2, add(5)(p15), add(7)(p14), add(7)(p14), add(8)(p12), add(9)(p22), add(10)(p13)x2, add(19)(q13) - matches published karyotype
Molec. Genetics:
Western blot: ER and AR positive; PR and HER2 negative (different to MCF-7)
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list