L-5178-Y
ACC 320
Cell line:
L-5178-Y
DSMZ no.:
ACC 320
Species:
murine (mouse) (Mus musculus)
Cell type:
T cell lymphoma
Origin:
established from an 8-month-old female DBA/2 mouse with T cell lymphoma in 1985
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at 0.1-0.5 x 106 cells/ml; split saturated culture 1:2 to 1:5 every 1-2 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 20 hours
Harvest:
max. density at about 2.0 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Cytogenetics:
murine near diploid karyotype with 13% polyploidy - 39(37-42)<2n> - 1 centric fusion marker
Viruses:
ELISA: reverse transcriptase positive; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list