SR-4987
ACC 323
Cell line:
SR-4987
DSMZ no.:
ACC 323
Species:
murine (mouse) (Mus musculus)
Cell type:
stromal cells
Origin:
established from the bone marrow of a female BDF/1 mouse in 1987; described in the literature to produce M-CSF, but not G-CSF, GM-CSF, or IL-3
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% McCoy's 5A + 10% h.i. FBS
Subculture:
split confluent culture 1:2 to 1:5 twice a week using trypsin/EDTA; seed out ca. 1.0-1.5 x 106 cells/25 cm2
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 24-36 hours
Harvest:
cell harvest of ca. 3.0-3.5 x 105/cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Cytogenetics:
murine hypotetraploid karyotype - 70(59-70)<4n> - 1-3 centric fusion, and a small ring, markers present
Viruses:
ELISA: reverse transcriptase negative; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list