Leibniz Institute DSMZ: Details

Cell line:

SGE-1

DSMZ no.:

ACC 343

Species:

rat (Rattus norvegicus)

Cell type:

kidney glomerular epithelial

Origin:

established from isolated renal glomeruli of a 3-week-old normal male Wistar rat in 1988

Reference(s):

15068, 15069

Biosafety level:

1

Permissions and restrictions:

A

DSMZ Cell Culture Data:

Morphology:

adherent cells growing as monolayer with polygonal shapes; image; image; image

Medium:

45% DMEM + 45% Ham's F12 + 10% h.i. FBS + 1x insulin-transferrin-sodium selenite + 10 ng/ml EGF

Subculture:

split culture prior to confluency, 1:10 to 1:20 twice a week using trypsin/EDTA; seed out at ca. 2-2.5 x 10⁴ cells/cm²; cells grow also with coating of the flask with collagen

Incubation:

at 37 °C with 5% CO₂

Doubling time:

ca. 33 hours

Harvest:

cell harvest of ca. 20 x 10⁶ cells/80 cm²

Storage:

frozen with 70% medium, 20% FBS, 10% DMSO

DSMZ Scientific Data:

Mycoplasma:

negative in DAPI, microbiological culture, RNA hybridization assays

Species validation:

Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Rattus norvegicus species.

Cytogenetics:

rat flat-moded hyperdiploid karyotype - 43-48<2n>

Viruses:

ELISA: reverse transcriptase positive; PCR: SMRV -

Supplied as:

Delivery form		Prices

Frozen culture		450,- €
Growing culture (please inquire for exact delivery time)		900,- €
DNA isolated from cell line (25 µg)		560,- €
DNA isolated from cell line (5 µg)		140,- €

see price list

Print data sheet