TFK-1
ACC 344
Cell line:
TFK-1
DSMZ no.:
ACC 344
Species:
human (Homo sapiens)
Cell type:
bile duct carcinoma
Origin:
established from the surgical specimen extracted from a 63-year-old man with extrahepatic bile duct carcinoma; described in the literature to express c-erbB-2 protein
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 1-2 x 106 cells/80 cm2; split confluent culture 1:3 to 1:5 every 2-3 days using trypsin/EDTA (for 10-15 min)
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 40 hours
Harvest:
cell harvest of ca. 10-30 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded hypertriploid karyotype with 20% polyploidy - 72(64-81)<3n>XY/XYY, +1, +3, +5, +5, +6, -9, +11, -13, -16, -18, +20, +2mar, del(1)(q21), del(1)(p11), i(1q), del(3)(p12p21)x2, add(5)(q35), i(5p)x2, inv(7)(q11q35), del(11)(p11)x1-2, i(15p), i(17p) - matches published karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list