MH-7777A
ACC 345
Cell line:
MH-7777A
DSMZ no.:
ACC 345
Species:
rat (Rattus norvegicus)
Cell type:
hepatoma
Origin:
established from the liver tumor of a Buffalo rat; tumor cells were propagated in the hind leg
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% DMEM + 10% horse serum + 4 mM L-glutamine + 10 nM insulin + 10 µM dexamethasone
Subculture:
split nearly confluent culture 1:4 every 3-4 days using trypsin/EDTA, cells should not be grown to full confluency as they tend to detach; seed out at ca. 3 x 106 cells/80 cm2
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 48 hours
Harvest:
cell harvest of ca. 1.5 x 107/80 cm2
Storage:
frozen with 70% medium, 20% horse serum, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Rattus norvegicus species.
Cytogenetics:
near-tetraploid rat karyotype with 17% polyploiy - 73-82<4n>
Viruses:
ELISA: reverse transcriptase negative
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list