LAT
ACC 349
Cell line:
LAT
DSMZ no.:
ACC 349
Species:
ovine (sheep) (Ovis aries)
Cell type:
aortic tissue cells
Origin:
established from the aortic tissue cells of a lamb; cells were described in the literature to be susceptible to Visna Maedi Virus and to be free of bovine viral diarrhoea virus (BVDV)
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
split near-confluent culture 1:5 every 4-5 days using trypsin/EDTA; seed out at ca. 1.5 x 106 cells/80 cm2; optimal density at 1.0-4.5 x 104 cells/cm2
Incubation:
at 37 °C with 5% CO2
Doubling time:
30-50 hours
Harvest:
cell harvest of ca. 5 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS and 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Ovis aries species.
Cytogenetics:
ovine diploid karyotype with 27% polyploidy - 54<2n>XY, no consistent abnormality detected
Viruses:
ELISA: reverse transcriptase positive; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list